Generator

Part:BBa_J100088:Design

Designed by: Ben Clarkson   Group: Campbell M Lab   (2012-07-18)


J100071+J10063


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1242
    Illegal AgeI site found at 2944
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 674
    Illegal SapI.rc site found at 2458


Design Notes

Promoter includes Cad1 and Cad2 binding sites. Coding sequence contains Luciferin Regenerating Enzyme and Luciferase coding sequences.


Source

Promoter from E. coli geonome, Green Firefly luciferase from L. Cruciata

References